Composite

Part:BBa_K3320016

Designed by: Joseana Mendes Comodaro   Group: iGEM19_Amazonas-Brazil   (2019-10-20)


Hypoxia Repressor Unit

This is a part from our AND Gate, called hypoxia repressor unit.


Usage and Biology

The hypoxia repressor unit is composed of the fdhF promoter, inducible in low concentrations of oxygen, expressing the Srp repressor protein, which represses the Srp promoter producing TetR that regulates the expression of BBa_K3320013 by Tet promoter. BBa K3320016 Map.png

Figure 1. BBa_K3320016 as insert.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 867
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None